ISSN Print Online
You are at: Home > List of Issue > Table of Contents > Abstract > Full Text
full text
 ORIGINAL ARTICLE
Role of the Hypothalamo-Pituitary-Adrenal Axis in the Modulation of Pollinosis Induced by Pollen Antigens

doi:10.2332/allergolint.09-OA-0133

Maki Hashimoto, Eisuke F Sato, Keiichi Hiramoto, Emiko Kasahara and Masayasu Inoue [About this authors]

ABSTRACT

Background: To clarify the mechanism of stress-induced modification of allergic diseases, we studied the effect of restraint stress on plasma levels of cytokines and the symptoms of pollinosis in mice.
Methods: The effects of restraint stress and the role of the hypothalamo-pituitary-adrenal axis (HPA-axis) in the development of pollen antigen-induced pollinosis were studied in control, hypophysectomized, adrenalectomized or ACTH-administered mice. Twenty days after sensitization, animals were subjected to mild restraint stress for 3 hours, and plasma levels of IFN-γ, IL-10, and IgE were measured. We analyzed the incidence of sneezing and nasal rubbing in the sensitized animals.
Results: Plasma levels of IL-10 and IgE increased in the sensitized animals with a concomitant increase in the incidence of sneezing and nasal rubbing. The increases in plasma IgE, IL-10 and the incidence of sneezing and nasal rubbing were suppressed by restraint stress. Adrenalectomy increased IFN-γ, inhibited the increase in plasma IL-10 and IgE, and suppressed the incidence of sneezing. In contrast, hypophysectomy increased plasma levels of IL-10, IFN-γ, and IgE and the incidence of sneezing. Intraperitoneal administration of ACTH decreased IL-10 in plasma but increased IFN-γ and suppressed the incidence of nasal rubbing.
Conclusions: The present findings show that the HPA-axis and ACTH play important roles in the regulation of plasma cytokines and IgE thereby modulating symptoms of pollinosis. The results also suggest that a mild restraint stress suppresses the increase in Th2-dependent cytokines and IgE to reduce the symptoms of pollinosis.


KEY WORDS:
ACTH, HPA axis, IgE, pollinosis, stress

Received: 22 June 2009.
Accepted: 27 October 2009.

Allergology International 2010; 59: 201-206

INTRODUCTION

The clinical symptoms of allergic diseases including pollinosis and asthma decrease the quality of life, affecting areas such as work productivity and school performance.1 The prevalence of allergic diseases including pollinosis and asthma has been increasing in various countries.2 Pollen from the Japanese cedar (Cryptomeria japonica) is one of the major allergens; more than 10% of Japanese have been suffering from pollinosis.2, 3 Pollinosis is a type I allergic disease mainly induced by Japanese cedar or Japanese cypress pollens and is characterized by an enhanced production of IgE, release of histamine and leukotrienes from mast cells, and infiltration of eosinophils in the nasal mucosa.

Various stresses have been reported to affect the symptomatic manifestations of allergic diseases, such as atopic dermatitis, asthma, and allergic rhinitis, by activating the HPA-axis that modulates the balance between Th1- and Th2-type immune responses.4-6 Both ACTH and glucocorticoids have been reported to play important roles in the modulation of stress responses and immunological reactions.7 ACTH has been known to inhibit INF-γ thereby promoting Th2-type immune reactions.8-12 Glucocorticoids have been reported to increase Th2-type cytokines but suppress Th1-type cytokines and enhance the Th2-type immune reactions including IgE production.13, 14 However, various stresses have been known to have different effects on symptoms of allergic diseases depending on their types, strength and timing.15-26 In fact, the symptoms of allergic dermatitis can be aggravated by a strong stress but suppressed by a mild stress.27, 28 To clarify the mechanism of stress-induced modification of allergic diseases, we studied the effect of restraint stress on plasma levels of various cytokines and the symptom of rhinitis observed in mice sensitized with pollen antigens.

METHODS

ANIMALS

Eight-week-old male BDF-1 mice (control) and hypophysectmized, adrenalectomized or sham-operated mice were purchased from Japan SLC (Shizuoka, Japan). These mice were housed under standard environmental conditions (3-6 mice per cage) at 22-24°C under a 12 : 12 hour light-dark cycle, and fed laboratory chow and water ad libitum. The adrenalectomized mice were given a half-saline solution. Animal experiments were carried out according to the Animal Care and Use Committee of the Osaka City University Medical School.

SENSITIZATION OF MICE

Mice were intraperitoneally injected with 1 μg of pollen allergen (Cry j 1, Hayashibara, Okayama, Japan) mixed with 0.3 ml of aluminum hydroxide gel (Sigma Chemical, St. Louis, MO, USA) on days 0 (first day) and 4. Then, 5 μg of pollen antigen was administered into the bilateral nasal cavities once per day (from day 10 to 16).

ADMINISTRATION OF ACTH

From day 0 to 21, mice were injected intraperitoneally with 0.3 ml of physiological saline containing 57 ng of ACTH 1-24 (Wako Pure Chemical Industries, Osaka, Japan) three times a week as described previously.29

STRESS EXPERIMENTS

On day 21, each mouse was placed for 3 hours in a 50 ml conical tube with multiple ventilation holes as described previously.30 The restraint stress allowed mice to rotate from a supine to prone position, but not to turn their heads toward their tails, not to take food and water. Control mice were not exposed to the restraint stress but deprived of food and water for the same duration.

ANALYSIS OF ANIMAL SYMPTOMS

Nasal rubbing and sneezing behaviors were observed on day 21 as described previously.31 Five μl of antigen (0.1 μg/mice) was bilaterally instilled into the nasal cavities. After challenging antigen, animals were immediately placed into the observation cage (one animal/cage, 11 × 6 cm), and the incidence of nasal rubbing and sneezing was counted for 5 minutes. To evaluate nasal rubbing, each time the animal rubbed or touched the area near the nose with their forepaws, it was counted as one event. Touches around the eyes and the mouth were disregarded.

BLOOD SAMPLES

On day 21, mice were anesthetized with ether (Wako Pure Chemical Industries), and blood samples were collected from the heart in heparinized tubes. Blood samples were centrifuged at 15000 g for 10 minutes. Then, plasma samples were stored at -20°C until use for the analysis.

ANALYSIS OF CYTOKINES, IgE AND HORMONES

Plasma levels of IFN-γ, IL-10, IgE, cortisol and ACTH were determined using IFN-γ and IL-10 ELISA kit (Pierce Biotechnology, Rockford, IL, USA), IgE EIA kit (Yamasa, Ciba, Japan), corisol EIA kit (Oxford Biomedical Research, Oxford, MI, USA) and ACTH ELISA kit (Phoenix Pharmaceuticals, Burlingame, CA, USA), respectively, according to the manufacture's instructions.

STATISTICAL ANALYSIS

The data were expressed as mean ± SD derived from 3-29 animals. A p-value of less than 0.05 was considered significant. The differences between the groups were analyzed by Student's t-test.

RESULTS

EFFECT OF SENSITIZATION ON PLASMA CYTOKINES, IgE AND CLINICAL SYMPTOMS

Number of sneezing and nasal rubbing increased markedly after sensitization of animals with pollen allergen. Sensitization significantly increased plasma levels of IL-10 and IgE without affecting IFN-γ levels (Table 1). Thus, sensitization with pollen antigen enhanced the production of IL-10 to shift the Th1/Th2 balance to Th2-dominant type reactions and enhanced the secretion of IgE from B cells, and finally induced clinical symptoms of rhinitis, such as sneezing and nasal rubbing.

EFFECT OF RESTRAINT STRESS ON PLASMA CYTOKINES, IgE AND CLINICAL SYMPTOMS

The restraint stress decreased the plasma levels of IgE and IL-10 without affecting levels of IFN-γ. Although plasma levels of both ACTH and cortisol remained unchanged after sensitization, they increased after receiving restraint stress both in control and sensitized groups. Under identical conditions, the incidence of both sneezing and nasal rubbing in the sensitized mice was decreased by restraint stress, suggesting that pollinosis was suppressed by a mild stress (Table 1).

EFFECT OF THE HPA AXIS ON THE SENSITIZED MICE

To elucidate the possible involvement of the HPA axis, we studied the effects of adrenalectomy and hypophysectomy on both the clinical status and plasma cytokines. The incidence of sneezing was decreased by adrenalectomy but increased by hypophysectomy. When mice were injected intraperitoneally with ACTH during sensitization, the incidence of sneezing remained unchanged (Fig. 1). Under the identical conditions, the incidence of nasal rubbing was not affected by either adrenalectomy or hypophysectomy but decreased significantly by ACTH administration (Fig. 2). The increase in plasma levels of IL-10 in the sensitized animals was enhanced significantly by hypophysectomy but suppressed by either adrenalectomy or the administration of ACTH (Fig. 3). The increase in plasma levels of IFN-γ in the sensitized animals was significantly enhanced by either adrenalectomy, hypophysectomy or the administration of ACTH (Fig. 4). The increase in plasma levels of IgE after sensitization was significantly decreased by adrenalectomy but enhanced by hypophysectomy. Under identical conditions, ACTH had no appreciable effects on IgE levels in plasma (Fig. 5).

DISCUSSION

Stress responses in animals can be categorized into three stages, eustress, resilience and distress, and immune reactions in each stage may be different depending on the stage.23 It is well known that symptoms of inflammatory diseases are affected by a variety of stressors. In fact, Chida et al.16 and Amano et al.28 reported that strong stress accelerated allergic diseases. On the other hand, Nishioka et al.19 reported that allergic diseases could be suppressed by exposure of animals to restraint stress. Although strong stress has been known to accelerate allergic diseases, it is not clear whether the aggravation depends on the increase or decrease of glucocorticoids. Therefore, biological effects of stress should be analyzed depending on the type, strength, and duration of exposure to stressors.

The present work shows that mild restraint stress elevated the plasma levels of ACTH and cortisol but decreased IL-10 and IgE levels without changing IFN-γ, and suppressed the symptoms of pollinosis as determined by the incidence of sneezing and nasal rubbing. Since the stress response could be mediated predominantly via the hypothalamo-pituitary-adrenal axis that involves secretion of ACTH and cortisol, the effect of ACTH on the symptoms of pollinosis was analyzed. We found that physiologically low levels of ACTH suppressed the symptoms of pollinosis. To study the mechanism by which ACTH suppressed the symptoms of pollinosis, we used animals that had received either hypophysectomy or adrenalectomy. The symptoms of pollinosis were suppressed by adrenalectomy but enhanced by hypophysectomy, suggesting the pivotal action of the two organs on modulation inflammatory reactions.

The present work also showed that adrenalectomy decreased the plasma levels of IL-10 and IgE, thereby suppressing the symptoms of pollinosis. These changes induced by adrenalectomy seem to reflect the decrease in plasma cortisol, a potent stimulant for the Th2-type immune reactions including pollinosis.5, 13 Preliminary experiments revealed that adrenalectomy decreased plasma levels of cortisol from 1.4 to 0.5 ng/ml. This observation is consistent with our hypothesis that plasma cortisol plays important roles in the enhanced secretion of IL-10 and IgE to aggravate symptoms of pollinosis.

It has been well documented that pituitary secretion of ACTH is enhanced in adrenalectomized animals by a feed-back mechanism that stimulates synthesis and secretion of POMC-related peptides.32-34 Consistent with this hypothesis, preliminary experiments in this laboratory showed that adrenalectomy increased the plasma levels of ACTH from 1.0 to 5.9 ng/ml. These observations suggest that the elevation of ACTH in plasma might be responsible for the mechanism by which adrenalectomy suppressed the symptoms of pollinosis.

To our surprise, the symptoms of pollinosis were aggravated by hypophysectomy that decreased plasma levels of both ACTH and glucocorticoids. This observation suggests that POMC-derived peptides, such as ACTH, and cortisol secreted from the adrenal gland seem to modulate the balance between Th1- and Th2-type immune reactions in a pivotal manner. To test this hypothesis, we analyzed the effect of ACTH administration on the plasma levels of Th1- and Th2-related cytokines and IgE. Immunological analysis revealed that administration of ACTH increased the plasma levels of IFN-γ but decreased IL-10 and the incidence of nasal rubbing without affecting IgE levels in plasma.

It has been postulated that both nasal rubbing and sneezing are induced predominantly by IgE and histamine-dependent type I reactions. However, not only histamine but also other ligands, such as serotonine, leukotriens, substance P and opiate peptides released from inflammatory and/or neuronal pathways, have been shown to induce nasal rubbing.28 Thus, it is not surprising that administration of ACTH decreased nasal rubbing without affecting the incidence of sneezing.

ACTH and/or POMC-related peptides have been known to stimulate melanocortin receptors expressed on plasma membranes of macrophages, lymphocytes and other immunocytes, thereby modulating immunological reactions.35-40 In fact, the present work shows that administration of ACTH stimulated Th1-type reactions (as judged from the elevation of IFN-γ) with concomitant suppression of both Th2-type reactions (as determined from the decrease in IL-10) and the symptoms of pollinosis presumably by interacting with melanocortin receptors on immunocytes. In this context, Johnson et al.12 reported that administration of 1 to 3 μM of ACTH suppressed the Th1-type reactions. However, a recent study showed that physiological levels of ACTH (1-100 nM) stimulated Th1-type reactions (as judged from the elevation of IFN-γ).41 This observation is consistent with the results obtained from animals administered with physiologically low levels of ACTH (65 nM). It has been well documented that Th1- and Th2-type immunocytes interact with each other to modulate immunological reactions. These observations suggest that ACTH directly stimulates Th1-type cells but inhibits Th2-type cells, thereby increasing IFN-γ secretion and decreasing IL-10 to suppress the symptoms of pollinosis. Since the secretion of glucocorticoids from the adrenal gland is one of the major responses to ACTH, the effect of cortisol might also play an important role in the modulation of allergic reactions. In this context, glucocorticoids have been known to inhibit Th1-type immunocytes but stimulate Th2-type cells.13 Thus, ACTH secreted from the pituitary gland and glucocorticoids derived from the adrenal gland might regulate the Th1/Th2 balance in a pivotal manner thereby modulating stress responses including allergic reactions. The pathophysiological significance of the pivotal modulation of immunological reactions by ACTH and glucocorticoids (and other ligands secreted from the pituitary and adrenal glands) should be studied further to elucidate the full scope of the effects of various stressors on the clinical symptoms of allergic diseases including pollinosis.


ACKNOWLEDGEMENTS

This work was supported by Special Coordination Funds for Promoting Science and Technology from the Ministry of Education, Culture, Sports, Science and Technology (16590252 and 14370062), and 21st Century COE Program "Base to Overcome Fatigue" supported by MEXT, Japan.


REFERENCES

1
Kaari J. The role of intranasal corticosteroids in the management of pediatric allergic rhinitis. Clin Pediatr (Phila)  2006; 45: 697-704.
Medline

2
Kozutsumi D, Tsunematsu M, Yamaji T, Murakami R, Yokoyama M, Kino K. Cry-consensus peptide, a novel peptide for immunotherapy of Japanese cedar pollinosis, induces Th1-predominant response in Cry j 1-sensitized B10.S mice. Biol Pharm Bull 2006; 29: 2506-9.
Medline Chemport

3
Okubo K, Gotoh M, Shimada K, Ritsu M, Okuda M, Crawford B. Fexofenadine improves the quality of life and work productivity in Japanese patients with seasonal allergic rhinitis during the peak cedar pollinosis season. Int Arch Allergy Immunol 2005; 136: 148-54.
Medline Chemport

4
Magnan AO, Mely LG, Camilla CA et al. Assessment of the Th1/Th2 paradigm in whole blood in atopy and asthma. Increased IFN-gamma-producing CD8 (+) T cells in asthma. Am J Respir Crit Care Med 2000; 161: 1790-6.
Medline Chemport

5
Viveros-Paredes JM, Puebla-Perez AM, Gutierrez-Coronado O, Sandoval-Ramirez L, Villasenor-Garcia MM. Dysregulation of the Th1/Th2 cytokine profile is associated with immunosuppression induced by hypothalamic-pituitary-adrenal axis activation in mice. Int Immunopharmacol 2006; 6: 774-81.
Medline Chemport

6
Priftis KN, Papadimitriou A, Nicolaidou P, Chrousos GP. The hypothalamic-pituitary-adrenal axis in asthmatic children. Trends Endocrinol Metab 2008; 19: 32-8.
Medline Chemport

7
Chrousos GP. The hypothalamic-pituitary-adrenal axis and immune-mediated inflammation. N Engl J Med 1995; 332: 1351-62.
Medline Chemport

8
Romagnani S. Regulation and deregulation of human IgE synthesis. Immunol Today 1990; 11: 316-21.
Medline Chemport

9
Gascan H, Gauchat JF, de Waal Malefyt R, Schneider P, Yssel H, de Vries JE. Regulation of human IgE synthesis. Clin Exp Allergy 1991; 21(Suppl 1): 162-6.
Medline

10
Chretien I, Pene J, Briere F, De Waal Malefijt R, Rousset F, De Vries JE. Regulation of human IgE synthesis. I. Human IgE synthesis in vitro is determined by the reciprocal antagonistic effects of interleukin 4 and interferon-gamma. Eur J Immunol 1990; 20: 243-51.
Medline Chemport

11
Heijnen CJ, Zijlstra J, Kavelaars A, Croiset G, Ballieux RE. Modulation of the immune response by POMC-derived peptides. I. Influence on proliferation of human lymphocytes. Brain Behav Immun 1987; 1: 284-91.
Medline Chemport

12
Johnson HM, Torres BA, Smith EM, Dion LD, Blalock JE. Regulation of lymphokine (gamma-interferon) production by corticotropin. J Immunol 1984; 132: 246-50.
Medline Chemport

13
Elenkov IJ, Chrousos GP. Stress Hormones, Th1/Th2 patterns, Pro/Anti-inflammatory Cytokines and Susceptibility to Disease. Trends Endocrinol Metab 1999; 10: 359-68.
Medline Chemport

14
Kimata H, Lindley I, Furusho K. Effect of hydrocortisone on spontaneous IgE and IgG4 production in atopic patients. J Immunol 1995; 154: 3557-66.
Medline Chemport

15
Ito Y, Mine K, Ago Y, Nakagawa T, Fujiwara M, Ueki S. Attack stress and IgE antibody production in rats. Pharmacol Biochem Behav 1983; 19: 883-6.
Medline Chemport

16
Chida Y, Sudo N, Sonoda J, Hiramoto T, Kubo C. Early-life psychological stress exacerbates adult mouse asthma via the hypothalamus-pituitary-adrenal axis. Am J Respir Crit Care Med 2007; 175: 316-22.
Medline Chemport

17
Bowers SL, Bilbo SD, Dhabhar FS, Nelson RJ. Stressor-specific alterations in corticosterone and immune responses in mice. Brain Behav Immun 2008; 22: 105-13.
Medline Chemport

18
Loureiro I, Wada CY. Influence of stress on IgE production. Physiol Behav 1993; 53: 417-20.
Medline Chemport

19
Nishioka K, Okano M, Ichihara Y, Ichihara N, Nishizaki K. Immunosuppressive effect of restraint stress on the initiation of allergic rhinitis in mice. Int Arch Allergy Immunol 2005; 136: 142-7.
Medline Chemport

20
Choi EH, Brown BE, Crumrine D et al. Mechanisms by which psychologic stress alters cutaneous permeability barrier homeostasis and stratum corneum integrity. J Invest Dermatol 2005; 124: 587-95.
Medline Chemport

21
Fukui Y, Sudo N, Yu XN, Nukina H, Sogawa H, Kubo C. The restraint stress-induced reduction in lymphocyte cell number in lymphoid organs correlates with the suppression of in vivo antibody production. J Neuroimmunol 1997; 79: 211-7.
Medline Chemport

22
Pincus-Knackstedt MK, Joachim RA, Blois SM et al. Prenatal stress enhances susceptibility of murine adult offspring toward airway inflammation. J Immunol 2006; 177: 8484-92.
Medline Chemport

23
Dhabhar FS, McEwen BS. Acute stress enhances while chronic stress suppresses cell-mediated immunity in vivo: a potential role for leukocyte trafficking. Brain Behav Immun 1997; 11: 286-306.
Medline Chemport

24
Flint MS, Tinkle SS. C57BL/6 mice are resistant to acute restraint modulation of cutaneous hypersensitivity. Toxicol Sci 2001; 62: 250-6.
Medline Chemport

25
Saint-Mezard P, Chavagnac C, Bosset S et al. Psychological stress exerts an adjuvant effect on skin dendritic cell functions in vivo. J Immunol 2003; 171: 4073-80.
Medline Chemport

26
Nakano Y. Effect of chronic topical exposure to low-dose noxious chemicals and stress on skin sensitivity in mice. J Occup Health 2007; 49: 431-42.
Medline Chemport

27
Buske-Kirschbaum A, Geiben A, Hollig H, Morschhauser E, Hellhammer D. Altered responsiveness of the hypothalamus-pituitary-adrenal axis and the sympathetic adrenomedullary system to stress in patients with atopic dermatitis. J Clin Endocrinol Metab 2002; 87: 4245-51.
Medline Chemport

28
Amano H, Negishi I, Akiyama H, Ishikawa O. Psychological stress can trigger atopic dermatitis in NC/Nga mice: an inhibitory effect of corticotropin-releasing factor. Neuropsychopharmacology 2008; 33: 566-73.
Medline Chemport

29
Zwermann O, Schulte DM, Reincke M, Beuschlein F. ACTH 1-24 inhibits proliferation of adrenocortical tumors in vivo. Eur J Endocrinol 2005; 153: 435-44.
Medline Chemport

30
Okuyama K, Ohwada K, Sakurada S et al. The distinctive effects of acute and chronic psychological stress on airway inflammation in a murine model of allergic asthma. Allergol Int 2007; 56: 29-35.
Medline

31
Takubo M, Inoue T, Jiang S et al. Effects of hop extracts on nasal rubbing and sneezing in BALB/c mice. Biol Pharm Bull 2006; 29: 689-92.
Medline Chemport

32
Birnberg NC, Lissitzky JC, Hinman M, Herbert E. Glucocorticoids regulate proopiomelanocortin gene expression in vivo at the levels of transcription and secretion. Proc Natl Acad Sci U S A 1983; 80: 6982-6.
Medline Chemport

33
Dallman MF, Jones MT, Vernikos-Danellis J, Ganong WF. Corticosteroid feedback control of ACTH secretion: rapid effects of bilateral adrenalectomy on plasma ACTH in the rat. Endocrinology 1972; 91: 961-8.
Medline Chemport

34
Eberwine JH, Roberts JL. Glucocorticoid regulation of pro-opiomelanocortin gene transcription in the rat pituitary. J Biol Chem 1984; 259: 2166-70.
Medline Chemport

35
Takeuchi S, Kudo T, Takahashi S. Molecular cloning of the chicken melanocortin 2 (ACTH)-receptor gene. Biochim Biophys Acta 1998; 1403: 102-8.
Medline Chemport

36
Lam CW, Perretti M, Getting SJ. Melanocortin receptor signaling in RAW264.7 macrophage cell line. Peptides 2006; 27: 404-12.
Medline Chemport

37
Adan RA, Gispen WH. Melanocortins and the brain: from effects via receptors to drug targets. Eur J Pharmacol 2000; 405: 13-24.
Medline Chemport

38
Getting SJ, Christian HC, Flower RJ, Perretti M. Activation of melanocortin type 3 receptor as a molecular mechanism for adrenocorticotropic hormone efficacy in gouty arthritis. Arthritis Rheum 2002; 46: 2765-75.
Medline Chemport

39
Getting SJ, Gibbs L, Clark AJ, Flower RJ, Perretti M. POMC gene-derived peptides activate melanocortin type 3 receptor on murine macrophages, suppress cytokine release, and inhibit neutrophil migration in acute experimental inflammation. J Immunol 1999; 162: 7446-53.
Medline Chemport

40
Akbulut S, Byersdorfer CA, Larsen CP, Zimmer SL, Humphreys TD, Clarke BL. Expression of the melanocortin 5 receptor on rat lymphocytes. Biochem Biophys Res Commun 2001; 281: 1086-92.
Medline Chemport

41
Johnson EW, Hughes TK Jr, Smith EM. ACTH enhancement of T-lymphocyte cytotoxic responses. Cell Mol Neurobiol 2005; 25: 743-57.
Medline Chemport

Full Text PDF
link to Full Text PDF
(247k)

get reader In order to look at PDF, please use "Adobe Reader", downloading.
Copyright © JAPANESE SOCIETY OF ALLERGOLOGY All rights reserved.